Research workers in Columbia University in New York identified in 1975 the chemical presence of AZADIRACHTIN , nimbin, salanin and numerous other molecules which are responsible for the major pesticidal properties of Neem seeds. In the same year the US Department of Agriculture at Beltsville and other USDA stations embarked on a research programe to understand the chemistry of AZA. The major reference to the work on AZA is that of Nakanishi published in 1975 and subsequently a five step procedure was described by David Schroeder and Koji Nakanishi entitled "A simplified isolation procedure for Azadirachtin" published in the Journal of Natural Products (volume 50, Mar-Apr 87).

Azadirachtin is the most potent tetranortriterpenoid isolated till date from Neem Seeds. It, together with other limonoids present in neem seeds has proven to be very effective against more than 300 insect species as antifeedant, repellent and as an insect growth regulator at the same time not affecting the life cycles of beneficial insects like honey bees, spiders and butterflies. Perhaps the only product presently available which does not build resistance in insects. Azadirachtin together with other constituents of neem seeds such as salannin, nimbin, nimbidin, meliantriol and a number of other limonoids exhibit insect repellent, antifeedent and insect growth regulator activities. Azadirachtin based pesticides are versatile, easily biodegradable, leaving no toxic residues and thereby keeping the environment clean and safe.

Thus Neem is:

• Bio degradable
• effective against a broad spectrum of insect pests
• It is eco-friendly
• Harmless to plants- Non phytotoxic
• Eco-friendly, maintain the stability of soil fertility.
• Growth regulation (eradication of pest)
• Greater production
• Compatible in nature
• Application is easy.
• Economical
• Better shelf-life compared to other bio pesticides